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| Product Number | Product Name | Molecular Formula | Cas. No. | Description | Notes | Alt. Name | Alt. Name 2 |
| HC 9070 | 7-O-Amino-4- methylumbelliferone |
C10H9NO3 | 99908-11-9 | Coumarin derivatives such as 7-O-Amino-4-methylumbelliferone have been shown to be useful in a simple spectroscopic assay for aldehydes in biologically relevant media.1 Condensation of this compound with aldehydes (e.g., formaldehyde) forms aldimines that are susceptible to elimination with Lewis bases such as bovine serum albumin (BSA), forming blue-fluorescent 4-methylumbelliferone (4-MU). The excitation and emission maxima for 4-MU are the same at pH 7.0 (water) and pH 10.3 (0.15 M glycine buffer).2 Maximum fluorescence was observed at 445 nm when excited at 365 nm, and the fluorescence intensity is 100 times as intense at pH 10.3 than at pH 7.0. 5 mg = 26.2 micromoles; 25 mg = 131 micromoles. Download a Product Information Sheet for HC 9070 here. 1. Salahuddin, S.; Renaudet, O.; Reymond, J.-L. Org. Biomol. Chem. 2004, 2, 1471-1475. 2. Strachan, R.; Wood, J.; Hirschmann, R. J. Org. Chem. 1962, 27, 1074-1075. | Useful in a simple spectroscopic assay for aldehydes in biologically relevant media. | 7-(Aminooxy)-4-methyl-2H-chromen-2-one | |
| HC 9080 | MMBC | C20H13NO7 | 137350-66-4 | There are several reagents available for the detection and determination of thiols by labeling with a fluorescent reagent.1 MMBC (Methyl maleimidobenzochromenecarboxylate, also known as ThioGlo® 1) was developed by Yang and Langmuir for the detection of thiols.2,3 The maleimide moiety is susceptible to conjugate addition by thiols, converting MMBC, which is essentially nonfluorescent, into highly fluorescent adducts. The advantages of MMBC include: (1) MMBC reacts quickly (ca. 1 min) with thiols under neutral conditions (pH 7.0-7.4). (2) The fluorescent thiol adducts of MMBC have emission maxima at relatively long wavelengths (513 nm), exhibiting high quantum yields. (3) MMBC itself exhibits very little fluorescence at this wavelength (ca. 40-60 times less fluorescent), thus obviating the separation of unreacted starting material from the mixture. (4) MMBC is relatively resistant to hydrolysis at neutral pH. Higher pH's should be avoided (e.g., = 8.0) due to maleimide hydrolysis and nonspecific reactions with amine residues.1 For more information, download a Product Information Sheet for MMBC here. 1. Review: Tyagarajan, K.; Pretzer, E.; Wiktorowicz, J. E. Electrophoresis, 2003, 24, 2348-2358. (Link) 2. Yang, J.-R.; Langmuir, M. E., J. Heterocyclic Chem. 1991, 28, 1177-1180. 3. ThioGlo is a registered trademark of Covalent Associates, Inc. | Useful in the detection and determination of thiol-containing proteins, enzymes, and peptides. MMBC, which is essentially nonfluorescent, reacts rapidly with thiols at neutral pH to afford highly fluorescent adducts. | ThioGlo®1; Methyl 10-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)-9-methoxy-3-oxo-3H-benzo[f]chromene-2-carboxylate | |
| HC 9090 | Pyrroloquinoline quinone (PQQ) | C14H6N2O8 | 72909-34-3 | Coenzyme tailored for efficient oxidation of methanol and formaldehyde in methylotrophic bacteria. | Methoxatin | 4,5-dioxo-4,5-dihydro-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylic acid | |
| HC 9095 | Luminol synthon N- hydroxysuccinimide ester |
C18H19N3O6 | 1111822-73-1 | Luminol is one of the most widely used chemiluminescent substrates, and luminol based indirect bioassays are linear and efficient. 3-Aminophthalimide derivatives are considered to be “pro-chemiluminescent” substrates since treatment with hydrazine forms a primary amine and free luminol.1 These derivatives are also highly fluorescent and can be used for dual fluorescence and chemiluminescence studies.
Our Luminol synthon NHS ester (HC 9095) is designed for post synthetic modification of an oligonucleotide which can then be used as a fluorescent tagged oligo, or treated with hydrazine for chemiluminescence studies. 1. Mavri-Damelin, D.; Wilden, J.; Mani, A.-R.; Selden, C.; Hodgson, H.J.F.; Damelin, L.H. Bioconjugate Chem. 2009, 20, 266-273. |
Luminol precursor | 4-Amino-2-(hexanoic acid-N-hydroxysuccinimide ester)-isoindol-1,3-dione | API-NHS ester |
| HC 9097 | Glutathione reductase probe | C14H11NO6 | 1173888-41-9 | Thiols are significant factors in many biological systems, and variations in cellular thiol levels have been associated with a number of disease states including psoriasis, cancer, and AIDS. For this reason, thiol probes that are fast, selective and sensitive are critical in the development of assays that hinge on thiol detection. Xi and co-workers1 have reported on the properties and utility of the coumarin-maleimide as a thiol probe. They have shown that it (HC 9097) is water soluble, has efficient PET quenching, and offers significant turn-on fluorescence in the presence of GSH or cys. They show the Glutathione reductase probe to be highly selective for thiols over competing amino acids, and demonstrated the high sensitivity for low levels of thiols. References 1. Yi, L.; Li, H.; Sun, L.; Liu, L.; Zhang, C.; Xi, Z.Angew. Chem. Int. Ed. 2009, 47, 4034-4037. | Fast thiol detection with a probe utilizing coumarin as the fluorophore and maleimide as the thiol acceptor. | ||
| LB 7100 | Loading Buffer | N/A | None | For additional information on fluorous affinity purification, click here. Consists of 5% N,N-dimethylformamide in 100 mg/mL aqueous sodium chloride. | Used for the fluorous affinity purification of oligonucleotides. | ||
| LK 4000 | 3-(3-Aminopropyl)solketal | C9H19NO3 | 131606-42-3 | ||||
| LK 4005 | 12-Amino-3,6,9-trioxadodecan-1-ol | C9H21NO4 | 49542-66-7 | ||||
| LK 4010 | Cholesteryl N-(15,16-Dihydroxy- 4,7,10,13-tetraoxahexadecyl) carbamate |
C40H71NO8 | None | ||||
| LK 4020 | Cholesteryl N-(16-O- (Dimethoxytrityl)-15-hydroxy- 4,7,10,13-tetraoxahexadecyl) carbamate |
C61H89NO10 | 869354-69-8 | ||||
| LK 4030 | 1,19-Diamino-4,7,10,13,16- pentaoxanonadecane |
C14H32N2O5 | 869308-34-9 | ||||
| LK 4040 | 6,7-Dihydroxy-4-oxaheptylamine | C6H15NO3 | 139755-70-7 | ||||
| LK 4050 | 15,16-Dihydroxy-4,7,10,13- tetraoxahexadecylamine |
C12H27NO6 | 869308-36-1 | ||||
| LK 4060 | N-(15,16-Dihydroxy-4,7,10,13- tetraoxahexadecyl)-2,4- dinitroaniline |
C18H29N3O10 | 869308-38-3 | ||||
| LK 4070 | N-(15,16-Dihydroxy-4,7,10,13- tetraoxahexadecyl) trifluoroacetamide |
C14H26F3NO7 | 869308-40-7 | ||||
| LK 4080 | O1-(Dimethoxytrityl) hexaethylene glycol |
C33H44O9 | 123706-69-4 | ||||
| LK 4090 | [6-((Dimethoxytrityl)oxy)hexyl][6'- hydroxyhexyl]disulfide |
C33H44O4S2 | 148254-18-6 | This product should be used within a few days of receipt. After storing for an extended period of time, even at -20 oC, the compond will equilibrate to a mixture of mono, ditritylated and detritylated diols. | |||
| LK 4100 | N-(16-((Dimethoxytrityl)oxy)-15- hydroxy-4,7,10,13- tetraoxahexadecyl)-2,4- dinitroaniline |
C39H47N3O12 | 869308-41-8 | ||||
| LK 4110 | O1-(Dimethoxytrityl)propane- 1,3-diol |
C24H26O4 | 110675-04-2 | ||||
| LK 4120 | [3'-O-(Dimethoxytrityl)propyl][3- hydroxypropyl]disulfide |
C27H32O4S2 | 132796-34-0 | ||||
| LK 4130 | O1-(Dimethoxytrityl) tetraethylene glycol |
C29H36O7 | 158041-84-0 | ||||
| LK 4140 | O1-(Dimethoxytrityl) triethylene glycol |
C27H32O6 | 146669-11-6 | ||||
| LK 4145 | Ethylenediamine tetraacetic acid triethyl ester |
C16H28N2O8 | 90359-20-9 | ||||
| LK 4150 | bis-(6-Hydroxyhexyl) disulfide |
C12H26O2S2 | 80901-86-6 | ||||
| LK 4160 | Bis(3-Hydroxypropyl) disulfide |
C6H14O2S2 | 30453-21-5 | ||||
| LK 4170 | 3-(3-Hydroxypropyl)solketal | C9H18O4 | 206999-64-6 | ||||