BlackBerry® Quenchers
Stable, long-wavelength quenchers of fluorescence
BlackBerry® Quencher 650 (BBQ-650®) is ideal for the quenching of long-wavelength reporter fluorophores, useful in various probe applications involving contact or FRET quenching.
Non-fluorescent, absorbs light at long wavelengths
• Useful absorbance between 550 and 750 nm.
• λmax varies from 650 to >690 nm in various oligonucleotides.
• Quenches long-wavelength fluorophores such as Cy3, TAMRA, Texas Red, Cy5, and Cy5.5.
Robust, stable to synthesis
• Ammonia stable for mild deprotection.
• Install at internal, 3', or 5' positions with standard solid-phase oligonucleotide synthesis.
Excellent contact-mode quencher
• Highly efficient quencher of FAM, Cy3, Texas Red, Cy5, and Cy 5.5 in contact quenching applications such as molecular beacons.
• Particularly high affinity for Cy dyes. Signal-to-background ratios of >90 and >88 for Cy5 and Cy5.5, respectively, were observed in molecular beacons.
Excellent FRET-mode quencher
• Excellent spectral overlap with long-wavelength fluorophores such as Texas Red, ROX, Cy5, and Cy 5.5, thus allowing efficient FRET quenching.
• Cy5.5 was quenched with 99% efficiency when separated from BBQ-650™ by ten base pairs (ca. 40 Å) in a non-contact hybrid probe.
BlackBerry® Quencher Products
|
Item
|
Catalog No.
|
Size/pack |
Price (USD)
|
BL-2010 |
|||
(Add "-E" to part number for Expedite columns, "-A" for ABI-style crimp columns)
|
200 nmol columns (pkg of 4) |
$75.00 |
|
1 µmol columns (pkg of 4) |
$210.00 |
||
100 mg |
$135.00 |
||
1 g |
$1050.00 |
||
BL-2020 |
|||
(Add "-E" to part number for Expedite columns, "-A" for ABI-style crimp columns)
|
200 nmol columns (pkg of 4) |
$80.00 |
|
1 µmol columns (pkg of 4) |
$230.00 |
||
100 mg |
$147.00 |
||
1 g |
$1140.00 |
||
BL-2030 |
|||
(Add "-E" to part number for Expedite columns, "-A" for ABI-style crimp columns)
|
200 nmol columns (pkg of 4) |
$75.00 |
|
1 µmol columns (pkg of 4) |
$210.00 |
||
100 mg |
$135.00 |
||
1 g |
$1050.00 |
||
BL-1010 |
|||
50 µmol |
$150.00 |
||
100 µmol |
$275.00 |
||
0.25 g |
$650.00 |
||
BL-1020 |
|||
50 µmol |
$160.00 |
||
100 µmol |
$280.00 |
||
0.25 g |
$675.00 |
||
BL-1030 |
|||
100 µmol |
$390.00 |
||
0.25 g |
$695.00 |
||
BL-3010 |
|||
5 mg |
$95.00 |
||
25 mg |
$375.00 |
BL-2040 |
|
50 mg |
$465.00 |
||
100 mg |
$710.00 |
BL-3030 |
|
5 mg |
$250.00 |
||
10 mg |
$425.00 |
BlackBerry® Quenchers: Covered under US Patent #7,879,986, Berry & Associates, Inc.
"BlackBerry" is a registered trademark of Berry & Associates, Inc.
"BBQ-650" is a registered trademark of Berry & Associates, Inc.
See Terms, Warranty, Legal page for important information about BlackBerry products.
"Cy", "Cy3", "Cy5", and "Cy5.5" are trademarks of GE Healthcare. "Texas Red" is a registered trademark of Molecular Probes. "ROX", and "TAMRA" are trademarks of Applied Biosystems, Inc.
©2001–2010 Berry & Associates, Inc. All rights reserved.