The Click-mates™ Collection
The Cu(I) catalyzed [3+2] azide-alkyne cycloaddition (CuAAC) also known as the Click reaction (Scheme I), has become an invaluable tool in biochemical research. This cycloaddition reaction is remarkably efficient and reliable even in the presence of the diverse array of functional groups found in DNA and the variety of scientifically intriguing ligation partners. Development of the click methodology has led to the production and study of many modified DNA oligonucleotides (ODNs) for biological as well as nanotechnological and surface oriented applications. The beauty of the click cycloaddition lies in the efficient formation of a non-toxic triazole from biological building blocks that have been modified with non-perturbing azides and unactivated alkynes.
Scheme 1: The CuAAC Reaction
Our Click-mates™ collection includes alkynes for the classic CuAAC, as well as the streamlined copper-free click modifications. The Click-easy™ alkynes can help you to simplify bioconjugation work by employing cyclo-alkynes for efficient uncatalyzed click ligation.
Click-mates™ Alkynes
- BA 0174 5-Propargyloxy-dU CEP
- BA 0308 5-Octadiynyl-dU CEP
- BA 0316 Alkynyl-modifier-C6-dT CEP
- PY 7712 5-(Propargyloxy)-2'-deoxyuridine
- PY 7713 5-(1,7-Octadiyn-1-yl)-2'-deoxyuridine
- PY 7714 5'-O-(Dimethoxytrityl)-5-(propargyloxy)-2'-deoxyuridine
- BA 0364 5-Octadiynyl-TMS-dU CEP
- BA 0365 5-Octadiynyl-TMS-dC CEP
- BA 0366 5-Octadiynyl-dC CEP
- BA 0369 5-Octadiynyl-TIPS-dU CEP
Click-easy™ Alkynes (For copper-free Click modifications)
- BA 0372 5'-Click-easy™ BCN CEP I
- BA 0373 5'-Click-easy™ BCN CEP II
- LK 4320 Click-easy™ BCN-N-hydroxysuccinimide ester I
- LK 4330 Click-easy™ BCN-N-hydroxysuccinimide ester II
- LK 4300 Click-easy™ MFCO-N-hydroxysuccinimide ester
- BA 0368 5'-Click-easy™ MFCO CEP
Click-mates™ Azides
- BT 1075 Desthiobiotin-TEG azide
- BT 1085 Biotin-TEG azide
- FC 8150 Folate-TEG azide
- FC 8160 Tocopherol-TEG azide
- FD 13005 Water soluble dansyl-TEG azide
- FF 6110 6-Carboxyfluorescein-TEG azide
- FF 6120 6-Carboxyfluorescein-dipivalate TEG azide
- LK 4270 Aminooxy-TEG-azide
- BL 3030 BBQ-650TM-TEG azide
- FF 6130 6-TET-TEG-azide
- LK 4310 Amino-TEG azide
- FC 8170 PQQ-TEG azide
- FC 8180 Cholesteryl-TEG azide
- DB 8010 Dabcyl-TEG azide
The purchase of some of these products for use in applications relating to copper catalyzed azide-alkyne cycloaddition chemistry (“Click Chemistry”) includes a limited, nontransferable license to intellectual property owned by TSRI to use this product solely for internal non-commercial research activities and specifically excludes clinical, therapeutic, or diagnostic use in humans or animals. Information regarding a license for commercial use in Click Chemistry may be obtained directly from The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla, CA 92037, or by contacting 858-784-8140 or click@scripps.edu.
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