Notes: Fluorescent cytidine analog.
Description: Fluorescent cytidine analog. Download a brief overview here.
The ribo version fluoresces at 385 nm (Bergstrom, D. E.; Inoue, H.; Reddy, P. A. J. Org. Chem., 1982, 47, 2174-2178; Bergstrom, D., et. al. Synlett, 1992, 179-188).
When incorporated into oligonucleotides, dF pairs with dG, resulting in a higher melting temperature (Inoue, H.; Imura, A.; Ohtsuka, E. Nucleic Acids Res., 1985, 13, 7119-7128). In triple helices, dF pairs well with dA (Staubli, A. B.; Dervan, P. B. Nucleic Acids Res. 1994, 22, 2637-2642). We offer the phosphoramidite version, which has been used for oligonucleotide synthesis (Durland, R. H.; Rao, T. S.; Jayaraman, K.; Revankar, G. R. Bioconjugate Chem., 1995, 6, 278-282), where it is known as "P", and serves as a fluorescent replacement of T in targeting AT base pairs during triplex formation.
We also offer the corresponding nucleoside (PYA 11100).