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BA 0253
Product Information Molecular Structure

Fluorescein II CEP

Notes: For the installation of fluorescein at the 5'-terminus of an oligonucleotide while allowing multiple additions or assaying the coupling step.

Description: For the installation of fluorescein internally or at the 5'-terminus of an oligonucleotide, the phosphoramidite "6-FAM" (5'-Fluorescein CEP, BA 0054), which does not bear a DMT group, is a popular choice. However, the lack of a trityl group precludes multiple additions or assaying the coupling step. We therefore offer Fluorescein II CEP ("6-FAM II", BA 0253), which features the same tether length as 6-FAM, but includes a DMT group.1 Use normally, but with a 15 minute coupling. For the highest yields, prepare the oligo DMT-on and remove the trityl group after cleavage and deprotection. The DMT may also be used to facilitate cartridge purification with on-column detritylation, e.g. with Fluoro-Pak columns.

We also offer Fluorescein III CEP ("6-FAM III", BA 0334) with a DMT on a 1,3-diol framework.

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1. 5'-Fluorescein II CEP incorporates 6-carboxyfluorescein. The 5-carboxyfluorescein isomer of this product (CA Reg. No. 144676-14-2) is also known. See: Theisen, P.; McCollum, C.; Upadhya, K.; Jacobson, K.; Vu, H.; Andrus, A. Tetrahedron Lett. 1992, 33, 5033-5036.

Fluorescein II CEP
Product Details Product Pricing

MF: C67H76N3O14P

MW: 1178.31

CAS NO: 1027512-13-5

Storage Conditions: -20 oC, dry, dark

Purity≥90%

Availability: 

Stock maintained. Ships within 1-3 days.

Alternate Name: 

6-FAM II
Unit Weight Unit Price Quantity
100 µmol $255.00
0.25 g $495.00
 
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