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BA 0287
Product Information Molecular Structure

Fmoc-amino-modifier-C6-dT CEP

Notes:  For incorporation of a nucleobase-tethered amine that can be deprotected prior to cleaving the oligonucleotide from the solid support.

Description: The (fluorenylmethyl)carbamoyl (Fmoc) group has been shown to be a useful amine protecting group for amine modification of oligonucleotides.1 It is removed during cleavage/deprotection with ammonium hydroxide. Alternatively, the Fmoc group can be removed before cleavage of the oligonucleotide from the solid support,2 e.g., with piperidine, simplifying the acylation process. After the acylation is complete, the labeled oligonucleotide can then be cleaved from the support and further deprotected with ammonium hydroxide.

For applications requiring a nucleobase-tethered amine at internal or 5' positions, we offer the new Fmoc-protected compound Fmoc-Amino-Modifier-C6-dT CEP (BA 0287), which offers the possibility of on-bead acylation as discussed above. It is an alternative to the venerable Amino-Modifier-C6-dT CEP (BA 0015), which bears a trifluoroacetyl (TFA) protecting group. The TFA group cannot be removed without cleavage of the oligonucleotide from the resin.

For more information on this product and its use, download a Product Information Sheet here.

For 3'-amino-modification involving Fmoc-deprotection, see BA 0299 and BA 0307.

(1) Nelson, P. S.; Kent, M.; Muthini, S. Nucl. Acids Res. 1992, 20, 6253-6259.

(2) For example, see: (a) Gartner, Z. J.; Kanan, M. W.; Liu, D. R. J. Am. Chem. Soc. 2002, 124, 10304-10306; see Supporting Information, p. 3. (b) Gartner, Z. J.; Tse, B. N.; Grubina, R.; Doyon, J. B.; Snyder, T. M.; Liu, D. R. Science 2004, 305, 1601-1605; see Supporting Online Material, p. 2.

Fmoc-amino-modifier-C6-dT CEP
Product Details Product Pricing

MF: C63H73N6O11P

MW: 1121.26

CAS NO: None Assigned

Storage Conditions: -20 oC, dry

Purity≥95%

Availability: 

Stock maintained. Ships within 1-3 days.

Unit Weight Unit Price Quantity
100 µmol $180.00
0.25 g $325.00
 
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