3'-Fmoc-amino-modifier CPG (1000 Angstrom)
Notes: Useful for the installation of an amino group at the 3'-terminus of an oligonucleotide.
Description: For the installation of an amino group at the 3'-terminus of an oligonucleotide, a solid-support-linked monomer with a protected amine and DMT-protected alcohol is required. The amine protecting group is typically removed and acylated with an appropriate NHS ester. The (fluorenylmethyl)carbamoyl (Fmoc) group has been shown to be useful as such an amine protecting group for amine modification of oligonucleotides.1 It is removed during cleavage/deprotection with ammonium hydroxide. Alternatively, the Fmoc group can be removed before cleavage of the oligonucleotide from the solid support, e.g., with piperidine, simplifying the acylation process. After the acylation is complete, the labeled oligonucleotide can then be cleaved from the support and further deprotected with ammonium hydroxide.2
Berry & Associates offers a version of such an Fmoc-protected amino-modifier for installation of an amino group at the 3'-terminus, i.e., 3'-Fmoc-amino-modifier CPG, in both higher- and lower-loaded versions, namely BA 0299 (ca. 70-80 µmol/g on 500 Angstrom CPG) and BA 0307 (ca. 35-45 µmol/g on 1000 Angstrom CPG). Both products feature a 7-atom spacer between the amino group and the O-DMT group.
If an Fmoc-protected amine modifier is required for internal or 5'-incorporation, please see Fmoc-amino-modifier-C6-dT CEP.
For more information on this product and its use, download a Product Information Sheet here.
(1) Nelson, P. S.; Kent, M.; Muthini, S. Nucl. Acids Res. 1992, 20, 6253-6259.
(2) (a) Gartner, Z. J.; Kanan, M. W.; Liu, D. R. J. Am. Chem. Soc. 2002, 124, 10304-10306; see Supporting Information, p. 3. (b) Gartner, Z. J.; Tse, B. N.; Grubina, R.; Doyon, J. B.; Snyder, T. M.; Liu, D. R. Science 2004, 305, 1601-1605; see Supporting Online Material, p. 2.