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BA 0315
Product Information Molecular Structure

Caged strand-breaker CEP

Notes: Once installed into an oligonucleotide, irradiation at 365 nm at neutral pH causes strand cleavage, leaving phosphate groups on both daughter strands. In contrast to other related monomers, a second step involving treatment with base is not required.

Description: 

The introduction of nicks in DNA may be accomplished using photolabile nucleotides or nucleotide surrogates, resulting in so-called "caged strand-breaks".1 Various non-nucleosidic monomers have been developed for this purpose.2-7 Caged Strand-Breaker CEP is the most advanced example of this strategy, allowing complete removal of the photolabile protecting group from both phosphorylated daughter strands and the avoidance of a second step involving base treatment.6

For information on the use of this product, download a Product Information Sheet for BA 0315.

1. Reviews: (a) Mayer, G.; Heckel, A. Angew. Chem. Int. Ed. 2006, 45, 4900-4921. (b) Young, D. D.; Deiters, A. Org. Biomol. Chem. 2007, 5, 999-1005. Tang, X. Dmochowski, I. J. Mol. BioSyst. 2007, 3, 100-110.

2. Urdea, M. S.; Horn, T. US Patent 5,258,506, Nov. 2, 1993.

3. Ordoukhanian, P.; Taylor, J.-S. J. Am. Chem. Soc. 1995, 117, 9570-9571.

4. Zhang, K.; Taylor, J.-S. J. Am. Chem. Soc. 1999, 121, 11579-11580.

5. Ordoukhanian, P.; Taylor, J.-S. Bioconj. Chem. 2000, 11, 94-103.

6. Zhang, K.; Taylor, J.-S. Biochemistry 2001, 40, 153-159.

7. Dell'Aquila, C.; Imbach, J.-L.; Rayner, B. Tetrahedron Lett. 1997, 38, 5289-5292.

Caged strand-breaker CEP
Product Details Product Pricing

MF: C45H49N4O9P

MW: 820.87

CAS NO: 1221188-51-7

Storage Conditions: -20 oC, dry, dark

Purity≥90%

Availability: 

Stock maintained. Ships within 1-3 days.

Alternate Name: 

3-O-[(Diisopropylamino)-(2-cyanoethoxy)phosphino]-1-
O-(4,4'-dimethoxytrityl)-1,3-di-(2-nitrophenyl)-1,3-
propanediol
Unit Weight Unit Price Quantity
100 µmol $340.00
0.25 g $795.00
 
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