(5'S)-8,5'-Cyclodeoxyadenosine CEP
Notes: This phosphoramidite is used for standard 3’ to 5’ oligonucleotide synthesis. Description: 8,5’-Cyclodeoxyadenosine (cyclo-dA) is a naturally occurring
free-radical-induced DNA lesion. This bulky lesion has been shown to be present
in human cells.1 Cyclo-dA is a strong block to
gene expression in both CHO and human cells but can be repaired via
nucleoside excision repair mechanisms, but not by base excision repair
mechanisms.2 Our (5'S)-8,5’-Cyclodeoxyadenosine CEP can be used for
efficient incorporation of cyclo-dA into oligonucleotides.
For additional information, download a Product Information
Sheet.
We also now offer the related THP protected cyclodeoxyguanosine analog. For information, see (5'S)-8,5'-Cyclodeoxyguanosine (THP) CEP (BA 0382).
References
(1) Dizdaroglu,
M.; Dirksen, M.L.; Jiang, H.X.; Robbins, J.H. J. Biochem. 1987, 241, 929-932.
(2) Brooks,
P.J.; Wise, D.S.; Berry, D.A.; Kosmoski, J.V.; Smerdon, M.J.; Somers, R.L.;
Mackie, H.; Spoonde, A.Y.; Ackerman, E.J.; Coleman, K.; Tarone, R.E.; Robbins,
J.H. J. Biol. Chem. 2000,
275 , 22355-22362.
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