Notes: Small, fluorescent natural base mimic that can signal the presence of abasic sites in hybridized DNA oligonucleotides.
BA 0346 is a phosphoramidite of a small, fluorescent nucleoside that is an isosteric mimic of thymidine.1,2 This probe has been shown to pair with adenine to form a stable duplex.
DNA probes constructed to contain BA 0346 at a selected sequence position show a significant emission enhancement when hybridization results in an opposing abasic residue as compared to an opposing adenine residue.1 This property makes BA 0346 useful for the preparation of probes that are designed to detect sequence-specific depurination and depyrimidination.
For more information, download a Product
Information Sheet for BA 0346 here.
1) Greco, N.J.; Tor, Y. J. Am. Chem. Soc. 2005, 127, 10784-10785.
2) Greco, N.J.; Tor, Y. Nature Protocols, 2007, 2, 305-316.